Biochemical and Biophysical Research Communications
Effect of metformin on adipose tissue resistin expression in db/db mice
Section snippets
Materials and methods
Animals. All animals were treated in accordance with the Animal Welfare Guideline of Akita University. Male obese diabetic db/db mice (C57BL/KsJ-leprdb/leprdb) and male C57BL/6J wild-type mice were obtained at 10 weeks of age from Nippon Clea (Tokyo, Japan). The mice were housed (n=3–4 per cage) in a room with relative humidity of 50% and a 12/12-h light/dark cycle at 20–22 °C, and allowed unrestricted access to standard rodent chow and water.
Metformin or insulin treatment. The db/db mice (10
Clinical characteristics
Table 1 shows the clinical characteristics of metformin-treated db/db mice (metformin group), insulin-treated db/db mice (insulin group), and vehicle-treated db/db mice (control group). As expected, fasting blood glucose and serum insulin levels were significantly reduced in the metformin group compared to the control group. In contrast to the metformin group, the insulin group exhibited high serum insulin levels, similar to the control group, although the insulin group showed a marked
Discussion
Originally, resistin was identified as an adipose-derived protein which may contribute to insulin resistance in murine models [7]. In the original paper, adipocyte resistin gene expression was shown to be downregulated by treatment with TZDs, and serum resistin levels were reported to be elevated in both genetic and diet-induced obese mice [7]. Therefore, we expected that resistin expression in adipose tissue would be reduced also by treatment with metformin, an insulin-sensitizing drug, in
Acknowledgements
This project was supported by a grant-in-aid from the Ministry of Education, Culture, Sports, Science and Technology of Japan (14770595).
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