Regular ArticleThe Sea Urchin sns Insulator Blocks CMV Enhancer following Integration in Human Cells
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Cited by (22)
The sea urchin sns5 insulator protects retroviral vectors from chromosomal position effects by maintaining active chromatin structure
2009, Molecular TherapyCitation Excerpt :Here, we investigated the ability of a new element, which is found in the early histone repeating unit of the sea urchin Paracentrotus lividus, to protect vector-encoded transgenes from chromosomal PE. We have previously identified an enhancer-blocker element of 265 bp, which has been termed sns, at the 3′ end of the H2A early histone gene that displays the capability to block enhancer-activated transcription in a polar and directional manner in both sea urchin and human cells.14,15,16 This element functions as an enhancer blocker in erythroid milieu and binds erythroid and ubiquitous transcription factors.17
Functional characterization of the sea urchin sns chromatin insulator in erythroid cells
2005, Blood Cells, Molecules, and DiseasesChromatin insulators and position effects
2003, New Comprehensive BiochemistryIdentification of the enhancer binding protein MBF-1 of the sea urchin modulator α-H2A histone gene
2002, Biochemical and Biophysical Research CommunicationsCitation Excerpt :Human U2-OS cells were cultured (at 37 °C in 5% CO2) in DMEM supplemented with 10% FBS (Euroclone). M30-EGFP DNA transfection and selection of clones were as described [21]. Clones were isolated, expanded, and transfected with the wild type and deletion mutants MBF-1 expressing plasmid.
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