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A Novel Early Estrogen-Regulated Gene gec1 Encodes a Protein Related to GABARAP

https://doi.org/10.1006/bbrc.2001.4908Get rights and content

Abstract

We have isolated, in guinea-pig endometrial cells, an estrogen-induced 1.8 kb RNA called gec1. Screening of a guinea-pig genomic library led to identification of gec1 gene consisting of 4 exons and 3 introns. Exon 1 contains the 5′UTR and the ATG initiation codon. A guinea-pig gec1 cDNA was obtained by 5′-RACE. The 351 bp coding sequence shares 76.8% identity with that of the human GABARAP 924 bp cDNA while UTRs of the two cDNAs differ. A gec1 probe from the 3′UTR revealed a 1.9 kb mRNA in human tissues and a human GEC1 cDNA was isolated from placenta. Its coding sequence shares 93 and 79% identity with that of guinea-pig gec1 and human GABARAP, respectively. The human and guinea-pig GEC1 proteins have 100% identity. GEC1 and GABARAP proteins have 87% identity and N terminus featuring a tubulin binding motif. Thus, estrogen-regulated gec1 is a new gene which could encode a microtubule-associated protein.

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    Sequence data from this article have been deposited with the EMBL/GenBank Data Libraries under Accession Nos. AF 012920 (guinea-pig gec1 cDNA), AF 312680 (guinea-pig gec1 genomic DNA), and AF287012 (human GEC1 cDNA).

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