Regular Article
Genetic Imbalances in Preleukemic Thymusesā˜†

https://doi.org/10.1006/bbrc.2001.4731Get rights and content

Abstract

To understand the molecular mechanisms involved in preleukemia, the suppression subtractive hybridization method was used in a murine radiation-induced thymic lymphoma model. Seventeen mRNAs overexpressed in preleukemic thymuses were identified: mouse laminin binding protein (p40/37LBP), E25 protein, Rattus norvegicus clone BB.1.4.1, profilin, poly(A) binding protein (PABP), mouse high mobility group protein 1, topoisomerase I, clusterin, proteasome RC1 subunit, rat prostatein C3 and C1 subunits; two ESTs and four unknown genes. The overexpression of PABP, clusterin, profilin, and the p40/37LBP mRNAs was confirmed in preleukemic thymuses and can be related to some cellular events observed during the preleukemic period, i.e., alterations of cell cycle and apoptosis properties. The p40/37LBP and 67-kDa laminin receptor proteins were upregulated during the preleukemic period. The data suggest that additional studies on p40/37LBP and 67-kDa laminin receptor regulation are required to evaluate their potential role in the lymphoma prevention by TNF-Ī± and IFN-Ī³.

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    ā˜†

    Abbreviations used: SSH, suppression subtractive hybridization; 37LBP, 37-kDa mouse laminin binding protein; 67LR, 67-kDa laminin receptor; PABP, poly(A) binding protein; EST, expressed sequence tags.

    1

    To whom correspondence should be addressed at CNCM, University of LiĆØge, Place Delcour 17, B-4000 LiĆØge, Belgium. Fax: 00 32 4 366 59 53. E-mail: [email protected].

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