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p21-Activated Kinase PAK Phosphorylates Desmin at Sites Different from Those for Rho-Associated Kinase

https://doi.org/10.1006/bbrc.2000.2854Get rights and content

Abstract

p21-activated kinase (PAK) and Rho-associated kinase (Rho-kinase) have been shown to induce Ca2+-independent contraction of smooth muscle. PAK-induced contraction of Triton-skinned smooth muscle correlates with increased phosphorylation of caldesmon and desmin, although the role of desmin phosphorylation has remained obscure. Here we report that desmin serves as an excellent substrate for PAK in vitro. PAK phosphorylated desmin in a GTP · Cdc42/Rac-dependent manner. Phosphorylation of desmin by PAK dramatically inhibited its filament-forming ability. PAK phosphorylated mainly serine residues of the head domain of desmin, and the major phosphorylation sites differed from those for Rho-kinase. These results suggest that different site-specific phosphorylation of desmin via two divergent protein kinases downstream of Rho family GTPases would seem to increase the regulatory potential for organization of desmin filaments.

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    Abbreviations used: PAK, p21-activated kinase; Rho-kinase, Rho-associated kinase; IF, intermediate filament; MBP, myelin basic protein; His6, 6 consecutive histidine; GST, glutathione S-transferase; λ-PPase, λ protein phosphatase; GTPγS, guanosine 5′-(3-O-thio)-triphosphate; PAGE, polyacrylamide gel electrophoresis; CBB, Coomassie Brilliant Blue.

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