Biochemical and Biophysical Research Communications
Diterpenes inhibit IL-12 production by DC and enhance Th2 cells polarization
Section snippets
Materials and methods
Culture medium, reagents, and monoclonal antibodies. The culture medium used in this study was serum-free AIM-V medium (Life Technologies, Paislex, UK). Recombinant human IL-4 (IL-4), recombinant human granulocyte-macrophage colony-stimulation factor (GM-CSF), CD40-L, rhIL-10, anti-IL10 mAb, rhIL-12, and anti-OX40L mAb were purchased from R&D Systems (Minneapolis, MN). LPS from Escherichia coli were purchased from Sigma–Aldrich (St. Louis, MO). For flow cytometry, monoclonal antibodies (mAbs)
Results and discussion
To investigate the direct effects of Sugiol or Secoferruginol on the function of human DC, immature monocyte-derived DC were exposed to Sugiol or Secoferruginol, and phenotypical and functional DC maturation were analyzed. Human monocytes were cultured with GM-CSF and IL-4 for 6 days under standard conditions, followed by another 2 days in the presence of various concentrations of Sugiol or Secoferruginol. The expression levels of CD1a, CD80, CD86, and HLA-DR as expressed by MFI on Sugiol- or
Acknowledgment
We thank Mrs. Annette Wallisch for critical reading of the manuscript.
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