Biochemical and Biophysical Research Communications
A novel ethylene receptor homolog gene isolated from ethylene-insensitive flowers of gladiolus (Gladiolus grandiflora hort.)
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Materials and methods
Plant materials and genomic DNA extraction. Gladiolus grandiflora hort cv. Traveler. (4x = 2n = 60) were grown in compost in a greenhouse condition. For expression analysis, flowers, at five distinct developmental stages from buds open to senescence, were collected and the different tissues were frozen in liquid nitrogen and stored at −80 °C until each analysis. Extraction of genomic DNA from young leaves of gladiolus was performed by DNeasy Plant Maxi Kit (Qiagen Co., Hilden, Germany) according to
Characterization of GgERS1a and GgERS1b
We isolated two gladiolus full-length cDNA homologues of the Arabidopsis ethylene receptor gene by 5′ and 3′ RACE-PCR. These two homologues have strong similarity to the amino acid sequences both of Arabidopsis thaliana AtERS1 as dicotyledon and Oryza sativa OsERS1 as monocotyledon (Fig. 1). One cDNA coding region was 1905 nucleotide in length and its open reading frame encoded 634 amino acids, and the other was 1269 nucleotide and 422 amino acids (Fig. 2A). The both deduced amino acid
Discussion
We isolated the GgERS1a and GgERS1b genes from a tetraploid plant, gladiolus. The predicted amino acid sequences of GgERS1a and GgERS1b were similar to that of Arabidopsis ERS1 gene encoding an ethylene receptor kinase [5], [6], [7]. The predicted GgERS1a protein was conserved at important domains such as TM, GAF, and HisKA, and at residues thought to be important for the normal function of ERS1 (residues Ala31, Ile62, Cys65, and Ala102, which correspond to the positions that are mutated in the
Acknowledgment
This work was supported by a research fellowship from the Japanese Society for Promotion of Sciences (JSPS), Japan.
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Cited by (0)
- 1
Present address: Division of Plant Physiology, Indian Agricultural Research Institute, New Delhi 110 012, India.
- 2
These authors equally contributed to this work.