Biochemical and Biophysical Research Communications
Different roles of histone H3 lysine 4 methylation in chromatin maintenance
Section snippets
Materials and methods
Yeast strains and plasmids. The yeast strains used in this study are summarized in Table 1. The histone H3 mutants were constructed by shuffling each plasmid containing histone H3 (WT or mutant alleles) and H4 into WZY42 (from Dent-Roth) to make YC85 (wild-type, pWZ414-F13), YC86 (pRS314-H3(K4A)-H4), YC87 (pRS314-H3(K36A)-H4), YC77 (pRS314-H3(K79A)-H4), and YC79 (pRS314-H3(K4A/K79A)-H4). Wild-type, K4A, and K36A mutant plasmids were gifts from Buratowski. An immunoblotting assay was performed
The expression of stress responsive genes is specific to the SET1 deletion
Deletion of SET1 increases the DNA repair capacities of the checkpoint mutants by inducing repair genes via Rad53 [25]. However, it is so far unclear which SET1 deletion is required for triggering the RAD53 dependent pathway. Possible explanations for this feature of SET1 deletion include loss of Set1 protein (regardless of HMT activity), loss of histone H3 K4 methylation, or loss of methylation of an unknown factor(s). As a first step toward clarifying this, the expression of several stress
Discussion
This study showed that set1Δ resulted in the induction of several stress responsive genes in a RAD53 dependent manner. Among the methylation targets of Set1, histone H3 K4 might be directly involved in this pathway, because a loss of histone H3 K4 methylation is necessary to induce the target genes. Interestingly, this pathway has differentiated the roles of H3 K4 methylation states. The loss of di- or tri-methylation, which is implicated in the active transcription, did not induce the stress
Acknowledgments
We thank Drs. S. Buratowski, A. Verreault, T. Kouzarides, S. Roeder, S. Dent-Roth, S.D. Briggs., S. Hanes, and B. Cairns for yeast strains and plasmids and A. Verreault for critical reading of the manuscript. This work was supported by the grant from the Korea Research Foundation Grant (R04-2004-000-10188-0, 2005-0497-000) to E.-J. Cho.
References (45)
Structure and dynamic behavior of nucleosomes
Curr. Opin. Genet. Dev.
(2003)- et al.
Methylation of H3-lysine 79 is mediated by a new family of HMTases without a SET domain
Curr. Biol.
(2002) - et al.
Disruptor of telomeric silencing-1 is a chromatin-specific histone H3 methyltransferase
J. Biol. Chem.
(2002) - et al.
Dot1p modulates silencing in yeast by methylation of the nucleosome core
Cell
(2002) - et al.
Methylation of H3 lysine 4 at euchromatin promotes Sir3p association with heterochromatin
J. Biol. Chem.
(2004) - et al.
Histone H3 methylation by Set2 directs deacetylation of coding regions by Rpd3S to suppress spurious intragenic transcription
Cell
(2005) - et al.
Cotranscriptional Set2 methylation of histone H3 lysine 36 recruits a repressive Rpd3 complex
Cell
(2005) - et al.
The Set2 histone methyltransferase functions through the phosphorylated carboxyl-terminal domain of RNA polymerase II
J. Biol. Chem.
(2003) - et al.
Dynamic lysine methylation on histone H3 defines the regulatory phase of gene transcription
Mol. Cell
(2005) - et al.
Molecular regulation of histone H3 tri-methylation by COMPASS and the regulation of gene expression
Mol. Cell
(2005)
Histone H2B ubiquitylation controls processive methylation but not mono-methylation by Dot1 and Set1
Mol. Cell
BUR kinase selectively regulates H3 K4 tri-methylation and H2B ubiquitylation through recruitment of the PAF elongation complex
Curr. Biol.
The Bur1/Bur2 complex is required for histone H2B mono-ubiquitination by Rad6/Bre1 and histone methylation by COMPASS
Mol. Cell
A Rad53 kinase-dependent surveillance mechanism that regulates histone protein levels in S. cerevisiae
Cell
AP1- mediated multidrug resistance in Saccaromyces cerevisiae requires FLR1 encoding a transporter of the mafor facilitator superfamily
J. Biol. Chem.
A novel membrane-bound glutathione S-Transferase functions in the stationary phase of the yeast Saccaromyces cerevisiae
J. Biol. Chem.
Purification and characterization of Saccharomyces cerevisiae DNA damage-responsive protein 48 (DDRP 48)
J. Biol. Chem.
Yeast carbamyl phosphate synthetase. Structure of the yeast gene and homology to Escherichia coli carbamyl phosphate synthetase
J. Biol. Chem.
Global loss of Set1-mediated H3 Lys4 tri-methylation is associated with silencing defects in Saccharomyces cerevisiae
J. Biol. Chem.
Histone H3 lysine 4 mono-methylation does not require ubiquitination of histone H2B
J. Mol. Biol.
Proteasomal ATPases link ubiquitylation of histone H2B to methylation of histone H3
Mol. Cell
The HORMA domain; a common structural denominator in mitotic checkpoints, chromosome synapsis and DNA repair
Trends Biochem. Sci.
Cited by (9)
MicroRNA let-7E in the mouse prefrontal cortex differentiates restraint-stress-resilient genotypes from susceptible genotype
2021, International Journal of Molecular SciencesAn enhanced isothermal amplification assay for viral detection
2020, Nature CommunicationsEpigenetics: spotlight on type 2 diabetes and obesity
2016, Journal of Endocrinological InvestigationLSD1 is essential for oocyte meiotic progression by regulating CDC25B expression in mice
2015, Nature Communications