Glycine receptor activation regulates short-term plasticity in CA1 area of hippocampal slices of rats
Section snippets
Materials and methods
The care and use of animals for these experiments followed the guidelines and protocols approved by the Institutional Animal Care and Use Committee of Institute of Neuroscience, Shanghai Institutes for Biological Sciences, the Chinese Academy of Sciences.
Preparation of slices. Wistar rats (14 to 21 days old) were decapitated, brains were quickly removed and 400 μm coronal hippocampal slices were cut in well-oxygenated ice-cold artificial cerebrospinal fluid (ACSF) using a vibratome, as
Glycine application increased PPR of PSCs
Postsynaptic currents were recorded from CA1 pyramidal neurons of hippocampal slices using the whole-cell patch–clamp technique. During the recording, a hyperpolarizing pulse (−5 mV) was employed to monitor the membrane conductance changes. The experiments were performed at −70 mV in the presence of Mg2+ to block the N-methyl-d-aspartate (NMDA) receptor-induced responses. Two consecutive stimuli were performed at IPI of 50 ms to study short-term plasticity.
To study the function of hippocampal
Discussion
In the present study, we have demonstrated that activation of GlyRs in CA1 area of rat hippocampal slices increases the PPR of PSCs induced by two consecutive stimuli, which depends on intact GABAAR activity. Our results reveal a possible role of GlyRs in hippocampal short-term plasticity through their influence on GABAAR-mediated synaptic currents.
GABAergic inhibition has been implicated in hippocampal short-term plasticity [22]. For example, the PPD of field potentials could be induced by
Acknowledgments
This study was supported by the National Basic Research Program of China (No. 2006CB500803) and the National Natural Science Foundation of China (Nos. 30125015 and 30321002) to T.-L. Xu. We thank Dr. B. Robertson for comments on the manuscript, and N. Gong for technical assistance.
References (26)
- et al.
Cellular and subcellular localization of the inhibitory glycine receptor in hippocampal neurons
Biochem. Biophys. Res. Commun.
(2004) - et al.
Shunting inhibition modulates neuronal gain during synaptic excitation
Neuron
(2003) - et al.
Information processing with frequency-dependent synaptic connections
Neurobiol. Learn. Mem.
(1998) - et al.
Short-term synaptic plasticity as a temporal filter
Trends Neurosci.
(2001) - et al.
Contributions of receptor desensitization and saturation to plasticity at the retinogeniculate synapse
Neuron
(2002) - et al.
Modulation of paired-pulse activation in the hippocampal dentate gyrus by cholecystokinin, baclofen and bicuculline
Neuropeptides
(1993) - et al.
Propofol effects on excitatory synaptic efficacy in the CA1 region of the developing hippocampus
Brain Res. Dev. Brain Res.
(2005) - et al.
Asymmetric cross-inhibition between GABAA and glycine receptors in rat spinal dorsal horn neurons
J. Biol. Chem.
(2003) - et al.
Local circuit plasticity in the rat dentate gyrus: characterization and aging-related impairment
Neuroscience
(2002) - et al.
Bridging the cleft at GABA synapses in the brain
Trends Neurosci.
(1994)
Morphologically identified glycinergic synapses in the hippocampus
Mol. Cell Neurosci.
Strychnine-sensitive glycine receptors depress hyperexcitability in rat dentate gyrus
J. Neurophysiol.
Pharmacological characterization of glycine-gated chloride currents recorded in rat hippocampal slices
J. Neurophysiol.
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2024, International Review of NeurobiologyCross-talk pattern between GABA<inf>A</inf>- and glycine-receptors in CNS neurons is shaped by their relative expression levels
2020, Brain ResearchCitation Excerpt :In the higher regions in the CNS, such as hippocampus and cortex, both GABAARs and GlyRs are expressed in the same neuron, but only GABAARs mediates fast inhibitory synaptic transmission. Glycine could be released and diffused from a specific set of neurons, astrocytes or blood vessels to GABAergic synapses, and thus constrains the strength of GABAergic synapses or regulates short-term plasticity (Zhang et al., 2006) through GlyRs-GABAARs cross-talk. The experimental protocols were approved by the Animal Care and Use Committee of the University of Science and Technology of China.
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2008, Brain ResearchCitation Excerpt :In the mature rat hippocampus, which expresses functional extrasynaptic GlyRs, there is increasing evidence that these extrasynaptic receptors play important roles in maintaining the normal excitatory balance for proper CNS function under physiological and/or pathological conditions (Chattipakorn and McMahon, 2003; Mitchell and Silver, 2003; Song et al., 2006; Zhang et al., 2008, 2006). Activation of GlyRs suppresses neuronal excitation and seizure-like events in the rat entorhinal cortex and hippocampus (Chattipakorn and McMahon, 2003; Kirchner et al., 2003; Song et al., 2006; Zhang et al., 2008, 2006). Meanwhile, the potent convulsant agent strychnine (STN) selectively antagonizes the GlyR, demonstrating the importance of GlyRs in CNS seizure activity.
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