Biochemical and Biophysical Research Communications
The N-terminal half of talin2 is sufficient for mouse development and survival
Section snippets
Materials and methods
Embryonic stem (ES) cells and mice. Talin2in1295 gene-trap SV129 ES cells (RRI434) were obtained from BayGenomics (UCSF) and injected into the C57BL/6 blastocysts. The resulting male chimeras were mated with C57BL/6 females, and the talin2in1295 line was subsequently maintained on a mixed C57BL/6:SV129 genetic background. Breeding was carried out under standard animal housing conditions in the UCD animal facility.
Genotyping was performed by polymerase chain reaction (PCR) assay. Mouse tail
Interruption of talin2 gene in mice
We have searched the available databases for mouse talin2 gene and two partial cDNA sequences (GenBank Accession Nos. AF467081 and BC059856) were identified. Together with the talin2 sequence from mouse chromosome 9 (XP_486227), we have assembled the mouse talin2 cDNA encoding 2453 amino acid residues, which shows 73% identity (85% similarity) with mouse talin1 (2541 amino acids, NM_011602) (Fig. 1).
By searching the BayGenomics database, we have found three gene-trapped embryonic stem (ES) cell
Discussion
In addition to the mouse, the existence of a second talin gene was reported in humans [12] and Dictyostelium[16]. We also found partial sequences of talin2 in chimpanzee (Pan troglodytes), rat (Rattus norvegicus), cow (Bos taurus), dog (Canis familiaris), red jungle fowl (Gallus gallus), and zebrafish (Danio rerio) from EST databases. Their derived amino acid sequences are extremely conserved. For example, human (NM_015059), rat (XP_236367), and mouse are 95% identical. However, the mammalian
Acknowledgments
We thank the BayGenomics for providing the gene-trapped ES clone. This study is supported in part by Shriner’s Children Hospital grant (S.H.L.).
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