Biochemical and Biophysical Research Communications
Free-soluble and outer membrane vesicle-associated VacA from Helicobacter pylori: Two forms of release, a different activity
Section snippets
Materials and methods
Bacterial strains, broth culture filtrate preparation, and OMV purification. Two well-characterized VacA-producing H. pylori strains (both with a s1a/m1 vacA genotype) were used: 60190 (ATCC 49503) and CCUG 17874 (from Culture Collection University of Göteborg, Göteborg, Sweden). VacA-containing broth culture filtrate (VacA+ BCF) was produced as described by Ricci et al. [19], [20]. Briefly, bacteria were grown in Brucella broth (Difco, Detroit, MI) supplemented with 1% Vitox (Oxoid,
Results and discussion
Mounting evidence suggests an important role for OMVs in the pathogenesis of bacterial diseases. OMVs are released by several Gram-negative bacteria, including Neisseria meningitidis, Haemophilus influenzae, Borrelia burgdorferi, Pseudomonas aeruginosa, Campylobacter jejuni, and enterotoxigenic Escherichia coli[16], [31], [32], [33]. OMVs, consisting of outer membrane lipids and a subset of outer membrane proteins and soluble periplasmic components, have been proposed to be vehicles for
Acknowledgments
This research was supported in part by grants from the Italian Ministry of University and Research (PRIN 2004 to V.R. and U.V.), from the Italian Ministry of Health to IRCCS Policlinico S. Matteo, and from the Pavia University.
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