Biochemical and Biophysical Research Communications
Protein-tyrosine kinase, Syk, is required for CXCL12-induced polarization of B cells☆
Section snippets
Materials and methods
Cells and cell culture. BAF3, a mouse IL-3-dependent pro-B cell line, and its mutant cell clones were cultured in RPMI1640 medium (Sigma, St. Louis, MO, USA) containing 10% fetal calf serum (FCS), 50 μM β-mercaptoethanol, and IL-3 in 5% CO2 humidified air at 37 °C.
Plasmids and transfection. Human syk cDNA, provided by Dr. Muller [11], was modified as previously described [12]. The PCR product of Flag-epitope-tagged (DYKDDDDK) human syk cDNA and the PCR product of Flag-tagged dominant-negative
CXCL12 induces tyrosine phosphorylation of Syk kinase
Previous studies have shown that CXCR4 signaling induced by CXCL12 participates in the retention of normal hematopoietic stem cells within the bone marrow [14], [15], [16], and Syk is potentially involved in hematopoiesis of the B cell lineage [17], [18]. To determine whether Syk is involved in hematopoiesis via CXCL12/CXCR4-induced signaling, BAF3 cells were stimulated with CXCL12, and tyrosine phosphorylation of whole cell lysates and Syk protein was analyzed. Rapid tyrosine phosphorylation
Acknowledgments
This study was supported in part by a Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science, the 21st Century COE Program of the Ministry of Education, and The Osaka Medical Research Foundation for Incurable Diseases.
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Cited by (22)
Phosphorylation of spleen tyrosine kinase at tyrosine 348 (pSyk<sup>348</sup>) may be a marker of advanced phase of Chronic Myeloid Leukemia (CML)
2015, Leukemia ResearchCitation Excerpt :However, for treating blast crisis, dasatinib (DAS) appears more attractive, owing to its large spectrum of target molecules. We have been interested in Syk expression and activation in CML because it is involved in different signaling pathways: (i) it potentially interacts with Src kinases, activated by BCR–ABL, and tyrosine kinase receptors [4,5]; (ii) it is involved in the molecular complexes activating actin and the cytoskeleton [6,7] and in integrin signaling pathways [8] regulating cell adhesion, a property that is impaired in CML [9]; (iii) it interacts with the PI3K/Akt pathway [10], activated by BCR–ABL [11]. We thought it interesting to assess the benefit of Syk phosphorylation as a biomarker, and as a potential therapeutic target during advanced phases (AP and BP) of CML.
Microenvironmental Interactions in Chronic Lymphocytic Leukemia: The Master Role of CD49d
2014, Seminars in HematologyBlockade of Syk ameliorates the development of murine sclerodermatous chronic graft-versus-host disease
2014, Journal of Dermatological ScienceCitation Excerpt :Chemokine stromal-derived factor 1 (SDF-1/CXCL12) and CXCR4 have been shown to play a crucial role in migration and development of hematopoietic stem cell transplantation [28]. Syk is required for CXCL12/CXCR4-induced cell polarization that occurs in concert with cell adhesion mediated by β-1 integrin [29]. There were higher expressions of CXCR4 on T cells, B cells, and CD11b+ cells after allogeneic BMT (18.60 ± 2.91%, 19.11 ± 2.17%, and 42.90 ± 4.17%) when compared with syngeneic group (2.61 ± 0.27%, 2.28 ± 0.50%, and 12.97 ± 0.64% with p < 0.01, p < 0.005, and p < 0.005, respectively, data not shown).
SYK regulates B-cell migration by phosphorylation of the F-actin interacting protein SWAP-70
2011, BloodCitation Excerpt :SYK is essential for B-cell development31 and required for immunoreceptor tyrosine-based activation-like motif (ITAM)–dependent F-actin assembly,32 but little is known of the requirement for SYK during recirculating B-cell migration, although it is required during chicken pre-B cell haptotactic migration in vitro.27 Furthermore, SYK is involved in the chemotaxis and adhesion of B-cell chronic lymphocytic leukemia (CLL) cells and in polarization of a pro-B cell line.33-35 The data presented here establish a role for SYK in regulating B-cell chemotaxis and adhesion, in part through phosphorylation of SWAP-70.
PRL-2 increases Epo and IL-3 responses in hematopoietic cells
2010, Blood Cells, Molecules, and Diseases
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Syk in cell polarity.