Biochemical and Biophysical Research Communications
Concentration-dependent collateral sensitivity of cisplatin-resistant gastric cancer cell sublines
Section snippets
Materials and methods
Cell culture and the selection of the gastric cancer cell sublines for cisplatin resistance. The human gastric cancer cell line, SNU-601, was obtained from the Cancer Research Center in Seoul National University (South Korea). The cells were cultured in RPMI 1640 (Gibco-BRL, Grand Island, NY, USA) supplemented with 10% FBS (Sigma Chemical, St. Louis, MO, USA). The cells were maintained as a monolayer culture and were subcultured at confluence. The cisplatin-resistant gastric cancer cell subline
Sensitivity of cisplatin-resistant gastric cancer cell sublines to various anticancer drugs
Of the eight human gastric cancer cell lines (SNU-1, SNU-5, SNU-16, SNU-484, SNU-601, SNU-620, SNU-638, and SNU-668), the SNU-601 cells were chosen owing to their having the lowest MT mRNA expression level. The cisplatin-resistant gastric cancer cell lines, SNU-601/Cis2 and /Cis10, which were selected by the chronic exposure to 2 and 10 μg/ml cisplatin, respectively, were 49 and >530 times more resistant to cisplatin than the parental SNU-601 cells (SNU-601/WT), respectively (Fig. 1). As shown
Discussion
The cisplatin analogs are among the most active and widely used cytotoxic anticancer drugs. However, the acquisition or presence of resistance significantly undermines the curative potential of these drugs against many malignancies [5]. In this study, two cisplatin-resistant gastric cancer cell sublines, SNU-601/Cis2 and /Cis10, were found to be resistant to cisplatin and various anticancer drugs including carboplatin, heptaplatin, mitomycin C, doxorubicin, and 5-fluorouracil. Interestingly,
Acknowledgments
This work was supported, in part, by grants from the Ministry of Science and Technology, Korea, and the Korea Science and Engineering Foundation through the Research Center for Resistant Cells (R13-2003-009).
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