Biochemical and Biophysical Research Communications
Clinico-molecular study of dedifferentiation in well-differentiated liposarcoma☆,☆☆
Section snippets
Materials and methods
Cases and methods of clinical study. Fifty cases in the WD and DD group treated in the Cancer Institute Hospital from 1978 to 2001 were studied. All samples were obtained from surgical specimens or biopsy specimens with no treatment by chemotherapy or radiotherapy.
Among the 50 cases, 5 cases had dedifferentiated lesions in the tumors. Clinical information such as localization, surgical margin, tumor size, clinical outcome, and pathological findings were examined for this study. Specimens had
Behavior of 50 well-differentiated/dedifferentiated type liposarcomas and relationship between dedifferentiation and tumor size
Fifty cases of well-differentiated and dedifferentiated type liposarcomas were studied. Out of the 5 DD cases, 2 had tumors located in the extremities and groin, respectively. The other 3 DD cases had tumors located in the retroperitoneum and died of disease after lung metastasis and local recurrences (Table 1A). The dedifferentiation of all 5 cases was aroused as a “de novo” lesion. No DD cases were seen with recurred lesions.
On the other hand, almost all WD cases had tumors located in the
Discussion
It is an interesting result that the well-differentiated lesions obtained from the DD cases showed a quite different gene expression profile from those of the WD cases by the unsupervised hierarchical clustering analysis. Although well-differentiated lesions from DD and WD are pathologically indistinguishable, our gene expression studies showed that the expression profiles are divergent.
The histological progression named dedifferentiation occurring in WD has been proposed to occur in a
Acknowledgements
This study has been supported by a Research Grant for the RIKEN Genome Exploration Research Project from the Ministry of Education, Culture, Sports, Science and Technologies of the Japanese Government and a Grant-in-Aid for Cancer Research from the Ministry of Health, Labour and Welfare of Japanese Government. We thank Manabe J, Kuroda K, Ae K, Tanizawa K, Tomaru Y, Hoshikawa Y, and Nagasaki K for helpful discussion.
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Abbreviations: WD, well-differentiated liposarcoma; DD, dedifferentiated liposarcoma; MX, myxoid liposarcoma; RC, round cell liposarcoma; LP, lipoma.
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Supplementary data associated with this article can be found in the online version, at doi: 10.1016/j.bbrc.2003.12.203.
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Present address: Division of Functional Genomics & Systems Medicine, Research Center for Genomic Medicine, Saitama Medical School, 1397-1 Yamane, Hidaka City, Saitama 350-1241, Japan.