Regular ArticleSpatial Distributions of Sulfur-Rich Proteins in Cornifying Epithelia
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Three-dimensional elemental mapping of phosphorus by quantitative electron spectroscopic tomography (QuEST)
2007, Journal of Structural BiologyNew arrangement of proteins and lipids in the stratum corneum cornified envelope
2007, Biochimica et Biophysica Acta - BiomembranesCitation Excerpt :This fact would involve an additional link of these lipids with the amino acids of the CE proteins, and hence, a specific distribution of the proteins sited on the extracellular surface of the CE. Several publications have focussed on the protein composition and structure of the CE [11–15]. Protein precursors of the CE have been shown to include involucrin [16] and loricrin [17,18].
Quantification and thickness correction of EFTEM phosphorus maps
2007, UltramicroscopyLocalizing Specific Elements Bound to Macromolecules by EFTEM
2007, Methods in Cell BiologyCitation Excerpt :For most applications, it is necessary to use freeze‐substituted plastic‐embedded sections that are unstained (Müller‐Reichert et al., 2003) or freeze‐dried cryosections (Leapman and Ornberg, 1988; Somlyo et al., 1979); frozen‐hydrated specimens are generally unable to withstand the required electron dose unless only very low spatial resolution information is sought. By using EFTEM, it is possible to detect physiologically relevant levels of phosphorus, sulfur, and certain other elements in cellular regions that are a few nanometers in size (Bazett‐Jones et al., 1994; Goping et al., 2003; Leapman et al., 1997). For example, by imaging phosphorus it is feasible to map quantitatively the distribution of nucleic acid with a detection limit (standard deviation) of ∼20 base pairs.
M. DarmonM. Blumenberg, Eds.
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