Abstract
GABAA receptor-associated protein (GABARAP) is a 14-kDa cytoplasmic protein initially identified as a molecular chaperone for GABAA receptor in cortical neurons. However, evidence indicates that the function of GABARAP is much broader than a specific role in neuronal cells. As an initial step to define the biological role of GABARAP, we searched for binding partners of GABARAP using co-immunoprecipitation coupled with LC-MS/MS analysis. As a result, DEAD (Asp-Glu-Ala-Asp/His) box polypeptide 47 (DDX 47), recently identified as RNA helicase, is identified as a binding partner of GABARAP. An interaction between GABARAP and DDX 47 was further confirmed by yeast two-hybrid systems. Further, co-transfection of GABARAP and DDX47 cDNA into a tumor cell line induces apoptosis. This result may be the first evidence showing that GABARAP and DDX 47 are involved in the apoptotic process.
Similar content being viewed by others
References
L Chen H Wang S Vicini RW Olsen (2000) ArticleTitleThe gamma-aminobutyric acid type A (GABAA) receptor-associated protein (GABARAP) promotes GABAA receptor clustering and modulates the channel kinetics Proc. Natl. Acad. Sci. USA 97 11557–11562 Occurrence Handle10.1073/pnas.190133497 Occurrence Handle1:CAS:528:DC%2BD3cXnsF2qsLk%3D Occurrence Handle10984509
S Fields O Song (1989) ArticleTitleA novel genetic system to detect protein-protein interactions Nature 340 245–246 Occurrence Handle10.1038/340245a0 Occurrence Handle1:CAS:528:DyaL1MXltlShsL8%3D Occurrence Handle2547163
AC Gavin M Bosche R Krause P Grandi M Marzioch A Bauer J Schultz JM Ricjk AM Michon et al. (2002) ArticleTitleFunctional organization of the yeast proteome by systematic analysis of protein complexes Nature 415 141–147
F Green T O’Hare A Blackwell CA Enns (2002) ArticleTitleAssociation of human transferrin receptor with GABARAP FEBS Lett. 518 101–106 Occurrence Handle10.1016/S0014-5793(02)02655-8 Occurrence Handle1:CAS:528:DC%2BD38XjsVSrs7w%3D Occurrence Handle11997026
L Guarente (1983) ArticleTitleYeast promoters and lacZ fusions designed to study expression of cloned genes in yeast Methods Enzymol. 101 181–191 Occurrence Handle1:CAS:528:DyaL2cXhvVOhsA%3D%3D Occurrence Handle6310321
H Ito Y Fukada K Murata A Kimura (1983) ArticleTitleTransformation of intact yeast cells treated with alkali cations J. Bacteriol. 153 163–168 Occurrence Handle1:CAS:528:DyaL3sXht1alsLs%3D Occurrence Handle6336730
E Jankowsky A Jankowsky (2000) ArticleTitleThe DExH/D protein family database Nucleic Acids Res. 28 333–334 Occurrence Handle10.1093/nar/28.1.333 Occurrence Handle1:CAS:528:DC%2BD3cXhvVKjurw%3D Occurrence Handle10592265
C Klebig S Seitz W Arnold N Deutschmann M Pacyna-Gengelbach S Scherneck I Petersen (2005) ArticleTitleCharacterization of {gamma}-aminobutyric acid type A receptor-associated protein, a novel tumor suppressor, showing reduced expression in breast cancer Cancer Res 65 394–400 Occurrence Handle1:CAS:528:DC%2BD2MXns1KrsA%3D%3D Occurrence Handle15695379
WD Philips SC Froehner (2002) ArticleTitleGABARAP and GABAA receptor clustering Neuron 33 4–6 Occurrence Handle10.1016/S0896-6273(01)00569-4 Occurrence Handle11779472
RL Strausberg EA Feingold et al. (2002) ArticleTitleGeneration and initial analysis of more than 15,000 full-length human and mouse cDNA sequences Proc. Natl. Acad. Sci. USA 99 16899–16903 Occurrence Handle10.1073/pnas.242603899 Occurrence Handle12477932
H Wang FK Bedford NJ Brandon SJ Moss RW Olsen (1999) ArticleTitleGABA(A)-receptor-associated protein links GABA(A) receptors and the cytoskeleton Nature 397 69–72 Occurrence Handle10.1038/16264 Occurrence Handle1:CAS:528:DyaK1MXns1WrsQ%3D%3D Occurrence Handle9892355
Acknowledgement
This work was supported by the research fund of Hanyang University (HY-2004-S).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Lee, J.H., Rho, S.B. & Chun, T. GABAA receptor-associated protein (GABARAP) induces apoptosis by interacting with DEAD (Asp-Glu-Ala-Asp/His) box polypeptide 47 (DDX 47). Biotechnol Lett 27, 623–628 (2005). https://doi.org/10.1007/s10529-005-3628-2
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s10529-005-3628-2