Abstract
Because of the high temperature applied in the steam pretreatment of lignocellulosic materials, different types of inhibiting degradation products of saccharides and lignin, such as acetic acid and furfural, are formed. The main objective of the present study was to examine the effect of acetic acid and furfural on the cellulase production of a filamentous fungus Trichoderma reesei RUT C30, which is known to be one of the best cellulase-producing strains. Mandels’s mineral medium, supplemented with steam-pretreated willow as the carbon source at a concentration corresponding to 10 g/L of carbohydrate, was used. Four different concentration levels of acetic acid (0–3.0 g/L) and furfural (0–1.2 g/L) were applied alone as well as in certain combinations. Two enzyme activities, cellulase and β-glucosidase, were measured. The highest cellulase activity obtained after a 7-d incubation was 1.55 FPU/mL with 1.0 g/L of acetic acid and 0.8 g/L of furfural added to the medium. This was 17% higher than that obtained without acetic acid and furfural. Furthermore, the results showed that acetic acid alone did not influence the cellulase activity even at the highest concentration. However, β-glucosidase activity was increased with increasing acetic acid concentration. Furfural proved to be an inhibiting agent causing a significant decrease in both cellulase and β-glucosidase production.
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References
Lynd, L. R. (1996), Annu. Rev. 21, 403–465.
Bashir, S. and Lee, S. (1994), Fuel Sci. Technol. Int. 12, 1427–1473.
Wyman, C. E. (1995), Solar Eng. 1, 359–366.
von Sivers, M. and Zacchi, G. (1995), Bioresour. Technol. 51, 43–52.
von Sivers, M. and Zacchi, G. (1996), Bioresour. Technol. 56, 131–140.
Jurasek, L. (1979), Dev. Ind. Microbiol. 20, 177–189.
Saddler, J. N., Brownell, H. H., Clermont, L. P., and Levitin, N. (1982), Biotechnol. Bioeng. 24, 1389–1402.
Grethlein, H. E., Allen, D. C., and Converse, A. O. (1984), Biotechnol. Bioeng. 26, 1498–1505.
Olsson, L. and Hahn-Hägerdal, B. (1993), Process Biochem. 28, 249–257.
Palmquist, E., Hahn-Hägerdal, B., Galbe, M., and Zacchi, G. (1996), Enzyme Microb. Technol. 19, 470–476.
Szengyel, Z., Zacchi, G., and Réczey, K, (1997), Appl. Biochem. Biotechnol. 63–65, 351–362.
Clark, T. A. and Mackie, K. L. (1984), J. Chem. Technol. Biotechnol. 34B, 101–110.
Larsson, S., Reimann, A., Nilvebrant, N., and Jönsson, L. J. (1999), Appl. Biochem. Biotechnol. 77–79, 91–103.
Theander, O. and Åman, P. (1978), Swed. J. Agric. Res. 8, 189–194.
Eklund, R., Galbe, M., and Zacchi, G. (1988), Int. Symp. Alcohol Fuels 8, 101–105.
Eklund, R., Galbe, M., and Zacchi, G. (1995), Bioresour. Eng. 52, 225–239.
Palmqvist, E., Hahn-Hägerdal, B., Galbe, M., Larsson, M., Stenberg, K., Szengyel, Z., Tengborg, C., and Zacchi, G. (1996), Bioresour. Technol. 58, 171–179.
Hägglund, E. (1951), in Chemistry of Wood, Academic, New York, pp. 324–332.
Mandels, M. and Weber, J. (1969), Adv. Chem. Ser. 95, 391–414.
Réczey, K., Szengyel, Z., Eklund, E., and Zacchi, G. (1996), Bioresour. Technol. 57, 25–30.
Mandels, M., Andreotti, R., and Roche, C. (1976), Biotechnol. Bioeng. Symp. 6, 21–33.
Berghem, L. E. E. and Petterson, L. G. (1974), Eur. J. Biochem. 46, 295–305.
Lambert, R. J. and Stratford, M. (1999), J. Appl. Microbiol. 86, 157–164.
Taherzadeh, M. J., Gustafsson, L., Niklasson, C., and Lidén G. (1999), J. Biosci. Bioeng. 87, 169–174.
Sternberg, D. (1976), Biotechnol. Bioeng. Symp. 6, 35–53.
Mukhopadhyay, S. N. and Malik, R. K. (1980), Biotechnol. Bioeng. 22, 2237–2250.
Tangnu, S. K., Blanch, H. W., and Wilke, C. R. (1981), Biotechnol. Bioeng. 23, 1837–1849.
Chahal, P. S., Chahal, D. S., and André, G. (1992), J. Ferm. Bioeng. 74, 126–128.
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Szengyel, Z., Zacchi, G. Effect of acetic acid and furfural on cellulase production of Trichoderma reesei RUT C30. Appl Biochem Biotechnol 89, 31–42 (2000). https://doi.org/10.1385/ABAB:89:1:31
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DOI: https://doi.org/10.1385/ABAB:89:1:31