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Effects of CcpA against salt stress in Lactiplantibacillus plantarum as assessed by comparative transcriptional analysis

  • Genomics, transcriptomics, proteomics
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Abstract

Lactiplantibacillus plantarum is frequently exposed to salt stress during industrial applications. Catabolite control protein (CcpA) controls the transcription of many genes, but its role in the response to salt stress remains unclear. In this study, we used transcriptome analyses to investigate differences in the logarithmic growth phases of Lactiplantibacillus plantarum ST-III and its ccpA-knockout mutant when grown with or without salt and glycine betaine (GB). The deletion of ccpA significantly affected bacterial growth under different conditions. Among the comparisons, the highest proportion of differentially expressed genes (64%) was observed in the comparison between the wild-type and ccpA mutant grown with NaCl, whereas the lowest proportion (6%) was observed in the comparison between the ccpA mutant strain cultures grown with NaCl alone or with GB together. Transcriptomic analyses showed that CcpA could regulate GB uptake, activate iron uptake, produce acetyl-CoA, and affect fatty acid composition to maintain membrane lipid homeostasis in the adaptation of high-salinity conditions. Conclusively, these results demonstrate the importance of CcpA as a master regulator of these processes in response to salt stress, and provide new insights into the complex regulatory network of lactic acid bacteria.

Key points

• The absence of CcpA significantly affected growth of L. plantarum and its response to salt stress.

• CcpA regulates compatible solutes absorption and ions transport to resist salt stress.

• CcpA alters fatty acids composition to maintain membrane lipid homeostasis towards salt stress.

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All data generated or analyzed during this study are included in this published article (and its supplementary information files).

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Acknowledgements

This work was supported by Xinjiang Production and Construction Corps Southern Xinjiang Key Industry Innovation and Development Support Program Project (2018DB002) Research on key technologies and equipment research and development for quality and efficiency deep processing of red dates in Southern Xinjiang. RNA-seq was performed at the Chinese National Human Genome Center at Shanghai for Functional Genomics and Biochip.

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Contributions

CC, SSZ, LS, and KH conceived and designed research, and conducted experiments. JH, HBY, XHL, and HXT analyzed the data. CC, HYY, SSZ, and KH interpreted the data and wrote the manuscript. All authors read and approved the submitted manuscript.

Corresponding author

Correspondence to Li Shao.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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The authors declare no competing interests.

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Chen, C., Huang, K., Li, X. et al. Effects of CcpA against salt stress in Lactiplantibacillus plantarum as assessed by comparative transcriptional analysis. Appl Microbiol Biotechnol 105, 3691–3704 (2021). https://doi.org/10.1007/s00253-021-11276-0

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  • DOI: https://doi.org/10.1007/s00253-021-11276-0

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