Introduction

L-amino acid oxidase (LAAO; EC: 1.4.3.2) is a flavoenzyme that catalyzes the oxidative deamination of an L-amino acid to an α-keto acid with the generation of ammonia and hydrogen peroxide (Wellner and Meister 1961). Previously, we described the bioactivities of LAAOs, including their antibacterial activity (Kasai et al. 2015b) that can be inhibited by catalases, suggesting that their antibacterial potential is due to the hydrogen peroxide produced during the reaction.

Hydrogen peroxide is a powerful oxidizing agent that acts as an intracellular signaling molecule and is involved in the oxidative bursts of phagocytes, thereby leading to the elimination of invading microorganisms (Clifford and Repine 1982). Briefly, hydrogen peroxide is an antimicrobial agent that induces DNA damage and cell death and is attributed to direct cytotoxicity and generation of reactive oxygen species (ROS) (Ueda and Shah 1992). This molecule regulates cell proliferation, growth arrest, apoptosis, and necrosis in various organisms (Vatansever et al. 2013). Owing to their diverse properties, LAAOs hold potential applications in detection and treatment of several diseased conditions, such as microbial infections, cancer, and autoimmune diseases. In this review, we have elaborated the biochemistry of LAAOs and focused on their potential applications in the field of biomedicine. This comprehensive overview of the current state of the LAAOs studies may facilitate the development of novel strategies for the diseased conditions.

Biochemical features of LAAOs and their mechanism of action

LAAOs are found in various animal fluids, including serum, snake venom, sea hare ink, body surface mucus of the snail and fish, mammary milk, and tissue extracts (mammary gland, intestine, liver, and kidney). Reportedly, several LAAOs have characteristic functions and excellent bioactivity in various species (Table 1). Further, Fig. 1 illustrates the molecular phylogenetic tree of LAAOs from various organisms. LAAOs are involved in innate immunity with selective and specific antibacterial properties (Kasai et al. 2015a (Table 1 V1), Kasai et al. 2015b (Table 1 V1), Teixeira et al. 2016 (Table 1 M2), Lazo et al. 2017 (Table 1 G), Rey-Suárez et al. 2018 (Table 1 N), Salama et al. 2018 (Table 1 L), Elsheimer-Matulova et al. 2020 (Table 1 D), Soares et al. 2020 (Table 1 E)), antiparasites (Carone et al. 2017 (Table 1 H2), Wiezel et al. 2019 (Table 1 M1), Soares et al. 2020 (Table 1 E), Barbosa et al. 2021) and induce DNA fragmentation (Mukherjee et al. 2015, Burin et al. 2016a, 2016b, Machado et al. 2018 (Table 1 H2), Machado et al. 2019a, 2019b (Table 1 H2)), apoptosis (Mukherjee et al. 2015, Fung et al. 2015 (Table 1 O), Burin et al. 2016a, 2016b, Ribeiro et al. 2016, Tan et al. 2017, Carone et al. 2017 (Table 1 H2), Machado et al. 2018 (Table 1 H2), Mukherjee et al. 2018, Rey-Suárez et al. 2018 (Table 1 N), Bedoya-Medina et al. 2019 (Table 1 N)), regulation of immune responses (Cousin et al. 2015, Scarlata et al. 2015, Romagnani 2016, Bod et al. 2018, Ramspott et al. 2018 (Table 1 A), Aubatin et al. 2018 (Table 1 A), Elsheimer-Matulova et al. 2020 (Table 1 D), Sadik et al. 2020), and cell cycle arrest (de Melo Alves Paiva et al. 2011 (Table 1 F), Machado et al. 2019a, 2019b (Table 1 H2)).

Table 1 Bioactive properties of LAAOs from several sources
Fig. 1
figure 1

Phylogenetic tree of LAAO from different vertebrates. The tree is constructed by neighbor-joining (NJ) method using LAAO sequences with the bootstrap values indicated (1000 replicates). Bootstrap values (≥ 50) are shown on the branch points. Scale bar represents 0.1 nucleotide substitutions per site

The mechanism of LAAOs’ antibacterial action has been investigated extensively and the most prevalent mechanism is via generation of hydrogen peroxide, as described above. An early report showed that LAAO isolated from Crotalus adamanteus venom to be effective against bacteria (Skarnes 1970). Many previous reports have highlighted that the bactericidal activity of LAAOs is generally inhibited by catalases (Stiles et al. 1991, Stábeli et al. 2007, Nagashima et al. 2009 (Table 1 U), Kasai et al. 2015a (Table 1 V1), Soares et al. 2020 (Table 1 E)), thereby the antibacterial action of LAAOs is due to the generated hydrogen peroxide.

Mammalian LAAO, which is capable of generating ROS upon exposure to an aromatic and hydrophobic amino acid, such as L-Trp or L-Phe in the presence of oxygen, has been found in milk (cow, donkey, and mouse), expressed immune cells (human, mouse, and chicken). Mammalian LAAO, which has an immunoregulatory function, was first detected in murine B cells (Chu and Paul 1997) and has been known to inhibit T cell proliferation (Boulland et al. 2007), cytotoxicity, and IFN-γ production by tumor-infiltrating CD8+ T cells, which favors tumor escape. Human LAAO, encoded by interleukin (IL)-4-induced gene 1 (IL4I1), is secreted and expressed by macrophages (Mφ) and dendritic cells (DCs) stimulated by microbial-derived products or interferons, which are endowed with immunoregulatory properties, and block the growth of gram-negative Escherichia coli and gram-positive Staphylococcus aureus (Puiffe et al. 2013).

Interestingly, Il4I1 is found in the mammalian spermatozoa head (human, equine, and bovine), particularly in the acrosomal. The aromatic amino acids were substrates for equine sperm LAAO, eliciting the dose- and time-dependent generation of ROS via mechanisms that were enhanced by cell death (Aitken et al. 2015). The combination of aromatic amino acids and nonviable cells was also found to enhance the levels of lipid peroxidation in live spermatozoa. Houston et al. (Table 1 A) reported that the stimulation of LAAO activity results in the induction of several hallmarks of capacitation, including tyrosine phosphorylation of the sperm flagellum and concomitant activation of phospho-SRC expression, that are important in the regulation of growth and differentiation of eukaryotic cells (2015). In addition, stimulation of IL4I1 resulted in an increase in the levels of acrosomal exocytosis in both the presence and absence of progesterone stimulation via a mechanism that could be significantly reversed by the presence of catalase. Their results suggested that the existence of human spermatozoan LAAO has a potential role in driving the redox regulation of sperm capacitation and acrosomal exocytosis (Houston et al. 2015 (Table 1 A)).

On the contrary, mice have two isoforms of LAAO encoded by Lao1 or Il4i1 that are localized and expressed in lactating mammary glands during the lactation period and immune cells by microbial-derived products or interferons, respectively, similar to humans. LAO1 present in milk protects against bacterial infections by producing hydrogen peroxide (Sun et al. 2002 (Table 1 C), Nagaoka et al. 2009 (Table 1 C)). Recently, gut microbiota profiles were compared between wild type and Lao1-knockout mouse pups to determine whether LAO1 affects infant gut microbiota development via mother’s milk consumption (Shigeno et al. 2019). In particular, the gut microbiota of lactating Lao1-knockout pups was highly diverse, whereas those of the wild type were composed of only a few dominant bacteria, such as Lactobacillus sps. Furthermore, cross-fostering indicated that milk of wild type mothers has the ability to suppress this diversity in Lao1-knockout pups. Moreover, the stomach contents of pups fed milk containing LAO1 could produce hydrogen peroxide. They suggested that hydrogen peroxide is generated from the process of free amino acid metabolism in the mother’s milk and may play a key role in regulating initial acquisition and development of the gut microbiota. Conclusively, LAO1-containing milk protects organs from bacterial infection via innate immunity.

Snake LAAOs are a major component of their venom and exhibit bactericidal (Lazo et al. 2017 (Table 1 G), Salama et al. 2018 (Table 1 L), Rey-Suárez et al. 2018 (Table 1 N)), parasiticidal (Carone et al. 2017 (Table 1 H2), Wiezel et al. 2019 (Table 1 M1), Barbosa et al. 2021), and anticancer effects through caspase activation (Fung et al. 2015 (Table 1 O), Mukherjee et al. 2015, Burin et al. 2016a, 2016b, Carone et al. 2017 (Table 1 H2), Tan et al. 2017, Mukherjee et al. 2018, Machado et al. 2018 (Table 1 H2)). CR-LAAO, isolated from Calloselasma rhodostoma venom, has antitumor potential and induces acute inflammatory responses similar to mammalian LAAO in vivo, with recruitment of neutrophils and release of IL-6, IL-1β, leukotriene B4 (LTB4), and prostaglandin E2 (PGE2). In vitro, IL-6 and IL-1β production by peritoneal macrophages stimulated by CR-LAAO was dependent on the activation of the Toll-like receptors, TLR2 and TLR4 (Costa et al. 2017). CR-LAAO and several other LAAOs promote apoptosis of tumor cells mediated by the release of hydrogen peroxide and activation of immune cells, resulting in oxidative stress and production of cytokines, such as IL-6 and IL-1β, which trigger a series of events, such as activation of caspase (-8, -9, and -3) cascade, and modulate cell cycle delay or arrest in the G0/G1 and S phases of tumor cells (Costa et al. 2017). Moreover, CR-LAAO alters regulation of apoptosis by modulating expression of miRNAs related to anti-apoptotic genes (Bid, Bim, Bcl-2, Cip-2, c-Flip, and Mcl-1) in BCR-ABL+ cells (Burin et al. 2016a). They suggested that CR-LAAO is a potential tool for enhancing apoptosis-related miRNA expression in chronic myeloid leukemia therapy (Burin et al. 2016a; Burin et al. 2016b).

Previously, we reported that 2.5 μg/mL of psLAAO1 isolated from the flounder Platichthys stellatus, which favors L-Lys as a substrate, produced approximately 2.7 mM of hydrogen peroxide within 1 h in the bacterial culture media (Kasai et al. 2015a (Table 1 V1)). This result suggests that sufficient levels of hydrogen peroxide can be produced by the action of psLAAO1 during the early bacterial growth phase to kill the bacteria under liquid culture conditions. Interestingly, psLAAO1 and other LAAOs (Achacin, OH-LAAO, SSAP, and CC-LAAO) bind to the bacterial membrane, which leads to localized high concentrations of hydrogen peroxide and antibacterial action in or near the bacterial surface (Ehara et al. 2002 (Table 1 P), Lee et al. 2011, Kitani et al. 2008 (Table 1 X), Kasai et al. 2015a (Table 1 V1), Abdelkafi-Koubaa et al. 2016). In summary, LAAOs selectively and specifically bind to sensitive bacteria and exhibit their antibacterial effect by exposing the cells to high, localized concentrations of hydrogen peroxide near the bacterial surface.

Characterization, structural stability, and regulation by metal ions

Recently, it has been reported that snake LAAOs have unique characteristics, namely, wide range of optimal pH, resistance to high temperatures, and structural conformation for enzymatic stability via regulation by metal ions. LAAOs from the venom of Bothrops jararacussu (BjussuLAAO-II) and Bothrops moojeni (BmooLAAO-I) showed high enzymatic activity in a wide temperature range of 25 to 75 °C with broad range pH and high optimum temperature (pH 6.0 to 9.0 at 65 °C and pH 5.5 to 9.5 at 60 °C).

Metal ions (Zn2+, Al3+, Cu2+, and Ni2+) negatively modulate the enzymatic activity of the aforementioned LAAOs (Costa et al. 2018 (Table 1 I)). A homodimeric Cv-LAAOI from the venom of Cerastes vipera has antibacterial, antiproliferative, and cytotoxic effects via extensive hydrogen peroxide production and optimum enzymatic activity on L-Leu at 50 °C. Moreover, the enzymatic activity is enhanced by Mn2+, while Cu2+, Hg2+, Ni2+, and Co2+ have suppressive effects on the oxidative activity (Salama et al. 2018 (Table 1 L)). LAAO from Peruvian Bothrops pictus (Bpic-LAAO), which is a homodimeric glycoprotein, exhibited optimal activity at pH 8.5 and remained stable up to 55 °C (Lazo et al. 201 7 (Table 1 G)). The kinetic activity of Bpic-LAAO was inhibited by Zn2+, while Ca2+, Mg2+, and Mn2+ ions induced no significant change. Furthermore, enzymatic activity of Cc-LAAOI and Cc-LAAOII, purified from Cerastes Tomato venom, was increased by Mn2+, while Zn2+, Ni2+, Co2+, Cu2+, and Al3+ ions markedly inhibited the activities of both isoforms. Notably, the optimal temperature of both enzymes remains stable up to 70 °C (El Hakim et al. 2015). They suggested that the thermoactivity of Cc-LAAO is related to the hydrogen bond between Ser73 and Gly70, which is an external loop consisting of residues 64–73 for contact with the FAD cofactor and might act as an energy barrier to adopt an optimal conformation for the catalytic activity. Therefore, the optimal temperature for Cc-LAAO can be surpassed at elevated temperatures. Moreover, the crystal structure and molecular dynamics simulations of LAAO from the venom of Bothrops atrox (BatroxLAAO) presented a dimeric arrangement that can be stabilized by Zn2+ (Feliciano et al. 2017 (Table 1 F)). These results suggest that metal coordination is critical for dimerization and eventually rendering effective catalytic activity.

We recently reported that psLAAO1 pre-treated with a chelating agent showed transiently increased antibacterial activity that was either decreased or diminished after one or two freeze–thaw cycles. Moreover, the mutant constructs of His348, whose position is predicted to be a metal coordination site by homology modeling simulation, did not show antibacterial activity or Mg2+ coordination (Kasai et al. 2020 (Table 1 V1)), indicating that Mg2+ coordination is essential for the enzymatic activity and structural stability of psLAAO1 at His348. Metal detection assay revealed that pre-chelated psLAAO1 did not detect Zn2+, whereas 1 mole of the wild type or mutant constructs (His348Ala and His209Ala) detected approximately 0.5 mole of Zn2+. These results suggest that Zn2+ does not coordinate with monomeric psLAAO1 and that the metal ion probably inhibits its enzymatic activity. Therefore, metal removal can potentially increase the enzymatic activity or vulnerability of LAAOs via changes in structural complex and conformation.

Immunoregulatory responses mediated by LAAOs

Human Mφ and DCs are major producers of L-Phe-catabolized IL4I1 under inflammatory conditions; B cells also express IL4I1 in response to NF-κB-activating stimuli (Marquet et al. 2010). IL4I1 inhibits the proliferation of CD3-stimulated T cells with a similar effect on CD4+ and CD8+ T cells via mature DCs. Notably, IL4I1 exerts inhibitory effects via its oxidative activity; these effects are associated with a transient downregulation of T cell receptor zeta (TCRζ) expression (Boulland et al. 2007). According to another study, the expression of IL4I1 has been reported in Th17 cells and a subset of FOXP3+ regulatory T (Treg) to Th17 transdifferentiating cells under inflammatory conditions (Scarlata et al. 2015). Moreover, IL4I1 expression is restricted to cells that do not express Helios, a transcription factor that characterizes natural Treg (nTreg) cells, but Aiolos, which is involved in the differentiation of Th17 and induced Treg (iTreg) cells. Supposedly, the expression of IL4I1 in Th17 and iTreg cells may provide insights into approaches that aim to modulate the balance of these populations in different pathological conditions involving inflammation-mediated immunosuppression, such as rheumatoid arthritis, inflammatory bowel disease, and multiple sclerosis. In fact, previous reports have suggested that the human IL4I1 ortholog is located on chromosome 19q13.3–19q13.4 (Chavan et al. 2002) in a region that is a hot spot for autoimmune disease susceptibility in general, including rheumatoid arthritis, multiple sclerosis, insulin-dependent diabetes mellitus, and systemic lupus erythematosus (Mason et al. 2004 (Table 1 B)). Therefore, an alteration in expression of IL4I1 could contribute to autoimmune disease, and its expression screening is a potential biomarker of inflammation-mediated immunosuppression.

Recently, IL4I1, which uses L-Phe as well as L-Trp as a substrate, activates the aryl hydrocarbon receptor (AHR) through the generation of indole metabolites and kynurenic acid (KynA) (Sadik et al. 2020) where the latter constitutes an important endogenous AHR ligand (Platten et al. 2019). In humans, L-Trp catabolism is initiated by indole-amine-2,3-dioxygenase 1/2 (IDO1/2) or tryptophan-2,3-dioxgenase (TDO2). Cancers express high levels of IDO1 and TDO2, taking advantage of L-Trp catabolite-mediated AHR activation. The AHR–KynA axis enhances the malignant phenotype of cancer cells, particularly cancer cell motility. Moreover, AHR–KynA signaling suppresses T cell proliferation and function by inducing differentiation of Treg cells, expression of programmed cell death protein 1 (PD1) on CD8+ T cells, and recruitment of immunosuppressive tumor-associated macrophages. These results suggest that IL4I1 promotes tumor progression as a metabolic consequence of immune checkpoints.

Recently, Elsheimer-Matulova et al. (Table 1 D) reported that IL4I1 from ISA brown chicken (Gallus gallus domesticus) is expressed in the cecum of newly hatched chickens 4 days post-infection with Salmonella enteritidis serovar Enteritidis. Moreover, chicken IL4I1 was expressed in Mφ, granulocytes, phagocytes, and CD4+ and γδ T cells, while they were not detected in CD8+ T or B cells (2020). Notably, mutation of the gene encoding chicken IL4I1 in Mφ HD11 did not affect the enzyme’s bactericidal capacity against S. enteritidis ser. Enteritidis but negatively affected its oxidative burst after phorbol 12-myristate 13-acetate (PMA) stimulation. Their findings suggest that IL4I1 is not directly involved in the bactericidal activity of phagocytes but instead it is likely involved in the control of inflammatory responses and signaling to T and B cells.

In a recent study, an antibacterial LAAO from the serum of red-spotted grouper (Epinephelus akaara) showed a broad range of substrate specificity with aromatic and hydrophobic amino acids, similar to mammalian and snake LAAOs (Osaka and Kitani 2021 (Table 1 S)). Interestingly, the encoding gene was expressed at a low level in the kidney under normal conditions, while it was significantly upregulated by blood loss. On the contrary, Il4i1-knockout mice been exhibit an accelerated B cell egress from the bone marrow, resulting in the accumulation of peripheral follicular B cells (Bod et al. 2018) and present a higher serum level of natural immunoglobulins and self-reactive antibodies. Collectively, several LAAOs including IL4I1 may function as extreme responses and vicious circles of inflammation due to bacterial and viral infection in the peripheral or tissue environments, such as bacillemia, ischemic disease, and sepsis. These LAAOs prefer aromatic and hydrophobic amino acids for substrate specificity and are conserved in vertebrates through the process of evolution (Fig. 1).

Perspectives for possible biomedical applications

Previously, we reported that LAAOs have bioactive potential; they can produce hydrogen peroxide and can be used for developing drugs against community and health care-associated bacterial infections. Briefly, LAAOs show significant antibacterial activity against various pathogenic bacteria such as E. coli, Pseudomonas aeruginosa, Acinetobacter baumannii, Klebsiella pneumonia, Proteus mirabilis, Vibrio cholera, Yersinia enterocolitica, S. aureus, S. epidermidis, Enterococcus faecalis, and Streptococcus pyogenes that cause infections such as that of the urinary tract, bloodstream, and abdominal cavity, diarrhea, vomiting, intestinal inflammation, endocarditis, tympanitis, and conjunctivitis (Kasai et al. 2015b (Table 1 V1)).

Several studies have reported procedures for the production of recombinant LAAOs. For instance, fungal LAAO from Rhizoctonia solani (RsLAAO1) was expressed in E. coli BL21 Codon Plus (DE3) as a fusion protein with maltose-binding protein to induce solubility (Hahn et al. 2017a; Hahn et al. 2017b). Further, hcLAAO4, one of the fungal LAAOs from Hebeloma cylindrosporum, was expressed in E. coli or yeast (Pichia pastors) systems with His-Tag (Bloess et al. 2019; Heß et al. 2020). Moreover, L-Lys-specific LAAO isolated from the Siganus pramin (serum) or Platichthys stellatus (gill), which is an innate immune protein with antibacterial activity, was codon optimized for expression in insect cells (Sf9) or P. pastoris expression systems (Han et al. 2020, Kasai et al. 2020 (Table 1 V1)). These recombinant LAAOs can be easily high mass-produced by scale-up and display strong bioactivity via bactericidal, parasiticidal, and apoptotic effects, and bind to the surface of bacteria, similar to native LAAOs, suggesting potential use of LAAOs against clinical pathogens. Moreover, L-Lys α-oxidase (LysOX; EC: 1.4.3.14), a homodimeric flavoenzyme that has substrate specificity for L-Lys as the name suggests, was identified from Scomber japonicus as an apoptosis-inducing protein. This enzyme was used for L-pipecolic acid synthesis using an E. coli expression system that expressed both LysOX and Δ1-piperideine-2-carboxylate reductase (Tani et al. 2015 (Table 1 W)). L-pipecolic acid produced by the action of LysOX is a precursor of numerous natural and synthetic bioactive compounds that function as immunosuppressants, anticancer agents, and local anesthetics. Based on these findings, LysOX is being investigated as a new approach for the production of chemical intermediates or precursors.

L-Trp is not only important in protein synthesis but also as a precursor of various biologically active compounds via the kynurenine pathway, generating metabolites with crucial functions in neurotransmission and regulation of immune responses. Notably, the depletion of L-Trp facilitates tumor immune escape by inducing Treg cells and expression of inhibitory receptors IL3 and IL4 on DCs and downregulating T cell receptor ζ-chain (TCRζ) in CD8+ T cells (Brenk et al. 2009). L-Trp catabolic products downstream of IDO1/2 and TDO2 regulate immune cell function and promote cancer progression by activating AHR (Gutiérrez-Vázquez and Quintana 2018). As mentioned above, IL4I1 is expressed in Th17 cells and a subset of Treg to Th17 transdifferentiating cells under inflammatory conditions. Therefore, profiling IL4I1 expression in immune cells is a probable biomarker of the inflammatory grade and stage.

Hydrogen peroxide is a widely used antimicrobial agent; its efficacy has been demonstrated in several human viruses, among which coronaviruses have been found to be sensitive (Dev Kumar et al. 2020). Recently, several chemical agents used for viral decontamination could effectively inactivate human coronaviruses by surface disinfection procedures using 62–71% ethanol, 0.1% sodium hypochlorite, or 0.5% hydrogen peroxide for 60 s (Kampf et al. 2020). Previously, we described the antiviral activity of LAAOs demonstrated by various studies (Kasai et al. 2015b (Table 1 V1)). For instance, LysOX from the fungus Trichoderma viride inhibited the reproduction of the type I herpes simplex virus (Lukasheva and Berezov 2002). Additionally, LysOX was heterologously expressed by Streptomyces lividans TK24 (Amano et al. 2015). The enzymatic properties of the purified recombinant LysOX, such as substrate specificity and thermal stability, are same as those of native LysOX. LAAO from venom of the Chinese green tree viper Trimeresurus stejnegeri (TSV-LAO) also displayed dose-dependent inhibition of HIV-1 infection and replication. Notably, in the presence of catalase, TSV-LAO inhibited syncytium formation in a dose-dependent manner, whereas anti-HIV-1 activity was not observed with the exogenous addition of hydrogen peroxide (Zhang et al. 2003). These results suggest that the antiviral activity of TSV-LAO does not affect infected cells via generation of hydrogen peroxide but rather by intermediate product or substrate depletion. L-Lys excess is characterized by a significant increase in HIV-1 RNA copies in enriched peripheral blood mononuclear cell fractions of HIV-infected patients and the copy number did not increase after adding L-Lys with LysOX in the plasma samples (Butorov 2015). Therefore, we consider that LysOX is a suitable antiviral as well as antibacterial agent. However, optimization of high production and yield of bioactive recombinant LAAOs and regulation of their cytotoxicity need further research.

Conclusions

LAAOs show high potency and efficacy as treatment strategy for various diseases and as biomarkers of autoimmune diseases, tumor grade, and immune signaling responses. Selective, specific, and localized activities of LAAOs in the early response are the underlying reasons for their potential biomedical implications. Although we now know that the direct or indirect bioactivities of LAAOs are associated with the catabolism of specific substrates and the production of hydrogen peroxide, several aspects such as the regulation of their enzymatic activities and conformation, selective usage per substrate specificity, production of intermediate products, and immune regulatory mechanisms remain poorly understood. It is, therefore, imperative to channelize future research with a clinical approach rendering each LAAO as a therapeutic agent. Moreover, LAAOs for clinical therapy must not only fulfil improved in vivo production but also pH resistance, thermal stability, and regulated substrate specificity and immune responses. Elucidating the mechanisms of LAAO selectivity against bacterial infections and evaluating the enzyme’s effects on resistance, cytotoxicity, and genotoxicity must also be characterized to fully realize the therapeutic potential of these enzymes for biomedical applications.