Characterization of a novel marine unicellular alga, Pseudoneochloris sp. strain NKY372003 as a high carbohydrate producer
Section snippets
Used strain
Microalgal strains were isolated from seawater obtained from sites on the coast of Yakushima island, which is one of the Osumi islands in Kagoshima prefecture, Japan. Among 292 strains isolated, strain NKY372003 was selected as the highest carbohydrate producer for further study here. This strain was maintained in IMK medium (Nihon Pharmaceutical, Osaka, Japan) dissolved in artificial seawater in Erlenmeyer flasks (50 ml) at 100 μmol photons/m2/s and 30 °C under the shaking condition.
Microscopic analysis
The
Characterization of strain NKY372003 by genotypic and morphological analyses
As a result of the screening from 292 isolates, strain NKY372003 showed fast growth and the highest carbohydrate content. Then, we examined the genotypic and morphological characteristics of this strain. Strain NKY372003 was identified based on 18S rDNA sequences (DDBJ/EMBL/GenBank accession number: LC505539), and displayed 96.95% of sequence identity with the 18S rDNA sequence of the unicellular green alga, Pseudoneochloris marina. Microscopic analysis indicated that vegetative cells of strain
Acknowledgments
This work was partially supported by JSPS KAKENHI Grant-in-Aid for Scientific Research B [grant number 17H03465].
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