Biochemical and Biophysical Research Communications
Liposome-encapsulated peptide PDBSN ameliorates high-fat-diet-induced obesity and improves metabolism homeostasis
Section snippets
Author contributions
Xirong GuoLiling XuDan shenJianfang GaoJia XiaXingyun WangYahui ZhouLing Chen
Synthesis and encapsulation of peptides
PDBSN (GLSVADLAESIMKNL) and the scramble peptide (MNAVSLELADLGSKI) used in this study were synthesized by Shanghai Scientific Peptide Biological Technology Co., Ltd.
The encapsulation of peptides was accomplished by Nanjing Nanoeast Biotech Co., Ltd. In brief, dissolve 60 mg PC (phosphatidylcholine) + 30 mg DSPE-PEG2000-COOH + 10 mg cholesterol into 4 ml chloroform, then to ensure it absolute solution through ultrasound. Next, rotating the solution at 70 °C to remove residual chloroform and form
ELISA
The ELISA kits for adiponectin (CSB-E07272 m) and leptin (CSB-E04650 m) were purchased from Wuhan Huamei Biotechnology Co., Ltd (Wuhan, China). The ELISA kits for IL-6 (4352) and TNF-α (7999) were purchased from Jiangsu MeiMian Industrial Co., Ltd (Yancheng, China).
Liposome encapsulation and modification increase peptide stability and specificity
To improve PDBSN characterizations in vivo, we designed a strategy by encapsulation of peptide into liposome (encapsulation efficiency (EE%): 63.14%) attached with cell-penetrated peptide (CPP) (encapsulation efficiency (EE%): 42.88%) and visceral adipose tissue targeting peptide (CGLHPAFQC) (encapsulation efficiency (EE%): 69.71%) [6](Fig. 1A). Then, the hydrodynamic size and zeta potential were analyzed using DLS (dynamic laser scatter) and results showed an effective coupling of peptide (
Discussion
On the basis of previous in vitro studies of the peptide PDBSN, we further encapsulated the peptide into liposomes attached to two ligands (targeting ligand and cell-penetrating peptide) in the present study. Then, the classic HFD-induced obese model was employed to study the in vivo function of PDBSN. Interestingly, PDBSN was enriched in adipose tissue, especially visceral fat, and reduced adipose mass and improved glucose metabolism as well as lipid homeostasis. Furthermore, the AMPK signal
Ethics approval
Animal studies were approved by the Ethics Committee of Shanghai sity (Number: 2019-097-01, Date: Dec. 20, 2019). All procedures were in accordance with the 3R (reduction, refinement, replacement) recommendations for ethical conduct of animal experimentation described in the Basel declaration (https://www.basel-declaration.org/basel-declaration/).
Consent for publication & competing interests
All authors agree to publish and confirm that there are no conflicts of interest.
Availability of data and materials
The datasets during and/or analyzed during the current study are available from the corresponding author on reasonable request.
Declaration of competing interest
All authors declare that there are no conflicts of interest about the article “Liposome-encapsulated peptide PDBSN ameliorates high-fat-diet-induced obesity and improves metabolism homeostasis”.
Acknowledgements and funding
This study was supported by the National Natural Science Foundation of China (Grant No. 81670773), Nanjing Technological Development Program (201803012), Jiangsu provincial key research and development program (BE2018614), the 333 high level talents training project of Jiangsu Province.
References (15)
- et al.
Obesity epidemiology worldwide
Gastroenterol. Clin.
(2016) - et al.
A novel peptide suppresses adipogenic differentiation through activation of the AMPK pathway
Biochem. Biophys. Res. Commun.
(2019) - et al.
Adipogenesis and WNT signalling
Trends Endocrinol. Metabol.
(2009) - et al.
AMPK activation regulates apoptosis, adipogenesis, and lipolysis by eIF2α in adipocytes
Biochem. Biophys. Res. Commun.
(2006) - et al.
Identification and characterization of a small molecule AMPK activator that treats key components of type 2 diabetes and the metabolic syndrome
Cell Metabol.
(2006) - et al.
AMPK: an emerging drug target for diabetes and the metabolic syndrome
Cell Metabol.
(2009) - et al.
Worldwide trends in body-mass index, underweight, overweight, and obesity from 1975 to 2016: a pooled analysis of 2416 population-based measurement studies in 128· 9 million children, adolescents, and adults
Lancet
(2017)
Cited by (13)
Obesity wars: hypothalamic sEVs a new hope
2023, Trends in Molecular MedicineIridium(III) complexes entrapped in liposomes trigger mitochondria-mediated apoptosis and GSDME-mediated pyroptosis
2022, Journal of Inorganic BiochemistryCitation Excerpt :Due to passive targeting, liposomes can also reduce side effects by avoiding the accumulation of drugs at non-target sites [20]. Moreover, liposomes can also achieve active targeting by using surface modifications like peptides, monoclonal antibodies, aptamers [21–23]. On the other hand, literature reported that nanoparticles, with the size between 5 nm and 500 nm, have a relatively long circulation time in vivo [24].
Advanced drug delivery systems for targeting obesity
2022, Drug Delivery Systems for Metabolic DisordersTargeting Peptides: The New Generation of Targeted Drug Delivery Systems
2023, PharmaceuticsEncapsulated Peptides and Proteins with an Effect on Satiety
2023, NanomaterialsLow-Molecular-Mass Fragments of Collagen Improve Parameters Related to Mass and Inflammation of the Adipose Tissue in the Obese Rat
2023, Food Technology and Biotechnology
- 1
These authors contribute equally to this work.