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Characterization of Platelet-Rich Plasma/Gellan Gum Hydrogel Composite for Biological Performance to Induce Chondrogenesis from Adipose-Derived Stem Cells

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Abstract

In this experiment, hydrogel composite was developed with gellan gum (GG) and platelet-rich plasma (PRP) to induce chondrogenesis of adipose-derived stem cells (ADSCs) in the hydrated environment. FTIR, SEM, and compression test were carried out to characterize the fabricated material. Biocompatibility was analyzed by MTT assay. The morphology of the encapsulated ADSCs was observed under SEM. Chondrogenesis was examined by PCR. The chemical and morphological analysis displayed the appearance of PRP in the matrix. The mechanical property of the hydrogel decreased as the PRP was loaded but viability and chondrogenesis were significantly increased with the proper amount of the PRP. Overall, the composite of the appropriate incorporation of PRP in the GG can enhance the microenvironment for chondrogenesis and expected to have great potential in tissue engineering.

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Correspondence to Gilson Khang.

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Acknowledgments: This research was supported by the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (HI15C2996) and the International Research & Development Program of the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (NRF-2017K1A3A7A03089427).

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Kim, N., Choi, J.H., Choi, M.J. et al. Characterization of Platelet-Rich Plasma/Gellan Gum Hydrogel Composite for Biological Performance to Induce Chondrogenesis from Adipose-Derived Stem Cells. Macromol. Res. 28, 1098–1103 (2020). https://doi.org/10.1007/s13233-020-8155-4

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