Preparation of chitosan microcarriers by high voltage electrostatic field and freeze drying
Section snippets
Strategy of experimentation
Both artificial liver and liver transplantation require a large amount of hepatocyte source. Compared with traditional single-layer plates, microcarriers have good mass transfer ability and mechanical stability, and can provide a larger specific surface area for hepatocyte culture. In addition, the microcarriers provides a three-dimensional growth space for the cells, which are more conducive to protecting the normal function of the cells and facilitating high-quality, high-density culture of
Preparation of chitosan microcarriers
Through experience summarization and single factor experiments, the factors affecting the preparation of microspheres are voltage, frequency, propulsion speed, pulse, liquid-surface distance and solution concentration. According to the experimental results, a voltage of 55 kV, a frequency of 90 Hz, a propulsion speed of 90 mm/h, a pulse width of 6 ms, and a liquid surface distance of 25 mm were selected for the preparation of microcarriers. Orthogonal experiments were carried out on the
Discussion
Liver is a highly organized structure. Once the primary hepatocytes are removed from an organism, tissue-specific functions are rapidly lost. Artificial liver is important for the treatment of fulminant hepatic failure. High-density culture of hepatocytes is vital for the development of artificial liver. The emergence of various microcarriers has made it possible to culture large numbers of hepatocytes. Piskin et al. (14) prepared poly-hydroxymethylsiloxanes (PDMS-OH) microcarriers by
Acknowledgments
This work was supported by the National Natural Science Foundation of China, No. 51776130.
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