MiR-30 family members inhibit osteoblast differentiation by suppressing Runx2 under unloading conditions in MC3T3-E1 cells

https://doi.org/10.1016/j.bbrc.2019.11.057Get rights and content
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Highlights

  • Disuse osteoporosis is common in prolonged space flight due to reduction of mechanical stress on bone.

  • Runx2 is one of the most important osteoblast-specific transcription factors.

  • Several miR-30 family members increased significantly under unloading condition.

  • Several miR-30 family members inhibited the expression of Runx2 and osteoblast differentiation.

  • The knockdown of miR-30 family attenuated partly the unloading -induced inhibition of osteoblast differentiation .

Abstract

Disuse osteoporosis is common in prolonged therapeutic bed rest, space flight and immobilization due to limb fracture, which is related to reduction of mechanical stress on bone. Mechanical unloading can inhibit the differentiation of osteoblasts, but the detailed mechanism is still unclear. Runt-related transcription factor-2 (Runx2), is an important transcription factor, which plays a crucial role in disuse osteoporosis induced by unloading conditions. In this study, we found that Runx2-targeting mechano-sensitive miR-30 family members, miR-30b, miR-30c, miR-30d and miR-30e increased significantly, and were negatively correlated with the expression of Runx2 under unloading condition. Further studies found that the four miRNAs inhibited the expression of Runx2 and osteoblast differentiation under normal loading, and the knockdown of these miRNAs attenuated partly the inhibition of osteoblast differentiation induced by unloading condition in MC3T3-E1 cells. This study is the first to report miR-30 family members can regulate partly the dysfunction of osteoblasts under unloading condition, which is expected to be targets for the treatment of disuse osteoporosis.

Keywords

miR-30
Runx2
Osteoblast differentiation
Mechanical unloading

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These authors contributed equally.