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Streptomycin mediated biofilm inhibition and suppression of virulence properties in Pseudomonas aeruginosa PAO1

  • Applied microbial and cell physiology
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Abstract

Pseudomonas aeruginosa is known as an opportunistic pathogen whose one of the antibiotic resistance mechanisms includes biofilm formation and virulence factor production. The present study showed that the sub-minimum inhibitory concentration (sub-MIC) of streptomycin inhibited the formation of biofilm and eradicated the established mature biofilm. Streptomycin at sub-MIC was also capable of inhibiting biofilm formation on the urinary catheters. In addition, the sub-MIC of streptomycin attenuated the bacterial virulence properties as confirmed by both phenotypic and gene expression studies. The optimal conditions for streptomycin to perform anti-biofilm and anti-virulence activities were proposed as alkaline TSB media (pH 7.9) at 35 °C. However, sub-MIC of streptomycin also exhibited a comparative anti-biofilm efficacy in LB media at similar pH level and temperature. Furthermore, this condition also improved the biofilm inhibition and eradication properties of streptomycin, tobramycin and tetracycline towards the biofilm formed by a clinical isolate of P. aeruginosa. Findings from the present study provide an important insight for further studies on the mechanisms of biofilm inhibition and dispersion of pre-existing biofilm by streptomycin as well as tobramycin and tetracycline under a specific culture environment.

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Funding

This work was supported by the Marine Biotechnology Program (Grant number 20150220) funded by Ministry of Oceans and Fisheries, Republic of Korea. Also, this work was financially supported by the National Institute of Fisheries Science (Grant number R2019053), Republic of Korea.

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FK, JWL, JHL, and DTNP performed the experiment. FK, HWK, YKK, and YK designed the experiment and analyzed the data. All authors were involved in the writing and correction of the manuscript.

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Correspondence to Young-Mog Kim.

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This article does not contain any studies with human participants or animals.

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The authors declare that they have no conflict of interest.

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Khan, F., Lee, JW., Pham, D.T.N. et al. Streptomycin mediated biofilm inhibition and suppression of virulence properties in Pseudomonas aeruginosa PAO1. Appl Microbiol Biotechnol 104, 799–816 (2020). https://doi.org/10.1007/s00253-019-10190-w

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  • DOI: https://doi.org/10.1007/s00253-019-10190-w

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