Predictive selection and evaluation of appropriate functional peptides for intestinal delivery with a porous silica gel
Section snippets
Materials
Porous silica gel, SMB-100-5 was supplied by Fuji Silysia Chemical Ltd., Aichi, Japan. Heat-treated silica gel was created from SMB-100-5 by calcining at 600 °C for 2 h under an air atmosphere (FG31, Yamato Scientific Co., Ltd., Tokyo, Japan).
Peptide synthesis for the evaluation of adsorption properties of silica
Peptide arrays were synthesized using a cellulose membrane and a spot synthesizer (ASP222, Intervis, Cologne, Germany) as previously described (18). After punching, each of the resulting peptide-containing disks (peptide spots) was placed in a single well
Drawing a hexa-peptide color map and peptide screening
In our previous report, we prepared color maps in which degree of intestinal delivery were predicted and depicted as contour lines. In these maps, score values of 32 tri-, penta-, and hepta- peptides were depicted. In the present study, a new color map was drawn with hexa-peptide library in the same way. We selected 32 kinds of peptides with varying hydrophobicity and pI (Fig. 1A), conducted adsorption experiments, and calculated score values (Table S1). Based on these results, we drew a
Acknowledgments
This work was partially supported by Japan Society for the Promotion of Science (JSPS) KAKENHI (Grant Numbers: JP16H04575), Japan and Aichi Science and Technology Foundation (17J6401b), Japan. We would like to thank Editage for English language editing.
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