Inspecting the genome sequence and agarases of Microbulbifer pacificus LD25 from a saltwater hot spring
Section snippets
Bacterial growth and 16S rRNA analysis
The bacterial LD25 strain was isolated and purified from Zhaori Spring, Green Island, Taiwan, a world-class saltwater hot spring with temperature of approximately 60–70°C. LD25 was cultivated in marine agar medium (BCRC Medium 61) at 30°C for 3 days. The agarase, protease, gelatinase, esterase, cellulose, and xylanase, activities of LD25 were determined by agar plate method. To observe the clear zone of enzymatic activities, the agar media were utilized as follows. (i) Agarase: Observation of
Identification of LD25 by 16S rRNA
LD25 was isolated and purified from a saltwater hot spring in Green Island, Taiwan. Several enzymatic activities including agarase, amylase, cellulase, esterase, gelatinase, protease, and xylanase were determined by agar plate and broth method. The results revealed that LD25 exhibited significant activities in agarase, cellulase, and gelatinase, followed by xylanase, amylase, esterase, and protease.
To realize the LD25 strain, 16S rRNA sequencing and phylogeny were conducted (Fig. S1). The 16S
Discussion
Several studies on the analysis of Microbulbifer species including the characterizations of agarase, chitinase, esterase, alginate lyase, amylase, and carrageenase have been published 14, 25, 26, 27, 28. In this study, M. pacificus LD25 was isolated from a saltwater hot spring and exhibited various enzymatic activities. The draft genome sequence of M. pacificus LD25 was carried out and submitted to the NCBI database for further inspection of its functional proteins. Compared with the different
Acknowledgements
This work was supported by the Educational and Scientific Research Program for Young Scholar Sponsored by Educational Department of Fujian Province (JAT170715), Fujian, China, the Doctor Startup Fund Project of Xiamen Medical Colledge (K2016-31), Fujian, China, and Systematic Management and Industrialized Application of Bioresources (4/4) (107-EC-17-A-22-0525), Ministry of Economic Affairs, Taiwan.
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