Abstract
Objectives
To create a multifunctional medical material that combines the advantages of both nanofibers and macroyarns.
Results
A novel electrospinning-based approach was developed for creating polycaprolactone (PCL) nanofiber covered yarns (PCL-NCYs) in which polyglycolic acid multi-strand filaments (PGA-MFs) were used as the core. BALB/3T3 (mouse embryonic fibroblast cell line) cells were cultured on the PCL-NCYs substrate and cell morphology and proliferation were determined by methylthiazol tetrazolium (MTT) assay. Compared with PGA-MFs, PCL-NCYs had a higher porosity and tensile strength of 88 ± 8% and 348 ± 16 MPa and in particular, the porosity was four times higher. BALB/3T3 cells attached more easily onto the nanofiber structure and proliferated along the direction of nanofibers, indicating that PCL-NCYs can achieve better cell differentiation and proliferation.
Conclusions
PCL-NCYs can be created by combining electrospinning covering and textile twisting, and have better mechanical property and higher porosity, and can be used as a novel scaffold in tissue engineering.
Similar content being viewed by others
References
Horst M, Madduri S, Milleret V, Sulser T, Gobet R (2013) A bilayered hybrid microfibrous PLGA-acellular matrix scaffold for hollow organ tissue engineering. Biomaterials 34:1537–1545
Jayasinghe SN (2013) Cell electrospinning: a novel tool for functionalising fibres, scaffolds and membranes with living cells and other advanced materials for regenerative biology and medicine. Analyst 138:2215–2223
Jiang T, Carbone EJ, Lo WH, Laurencin CT (2015) Electrospinning of polymer nanofibers for tissue regeneration. Prog Polym Sci 46:1–24
Joseph J, Nair SV, Menon D (2015) Integrating substrateless electrospinning with textile technology for creating biodegradable three-dimensional structures. Nano Lett 15:5420–5426
Li WJ, Laurencin CT, Caterson EJ, Tuan RS, Ko FK (2002) Electrospun nanofibrous structure: a novel scaffold for tissue engineering. J Biomed Mater Res A 60:613–621
Li D, Pan X, Sun B, Wu T, Chen W, Huang C, Ke Q, EI-Hamshary HA, Al-Deyabd SS, Mo X (2015) Nerve conduits constructed by electrospun P(LLA-CL) nanofibers and PLLA nanofiber yarns. J Mater Chem B 3:8823–8831
Mandal BB, Kundu SC (2009) Cell proliferation and migration in silk fibroin 3D scaffolds. Biomaterials 30:2956–2965
Mouthuy PA, Zargar N, Hakimi O, Lostis E, Carr A (2015) Fabrication of continuous electrospun filaments with potential for use as medical fibres. Biofabrication 7:025006
Padmakumar S, Joseph J, Neppalli MH, Mathew SE, Nair SV, Shankarappa SA, Menon D (2016) Electrospun polymeric core–sheath yarns as drug eluting surgical sutures. ACS Appl Mater Interface 8:6925–6934
Sill TJ, von Recum HA (2008) Electrospinning: applications in drug delivery and tissue engineering. Biomaterials 29:1989–2006
Subbiah T, Bhat GS, Tock RW, Parameswaran S, Ramkumar SS (2005) Electrospinning of nanofibers. J Appl Polym Sci 96:557–569
Tan L, Hu J, Huang H, Han J, Hu H (2015) Study of multi-functional electrospun composite nanofibrous mats for smart wound healing. Int J Biol Macromol 79:469–476
Townsend-Nicholson A, Jayasinghe SN (2006) Cell electrospinning: a unique biotechnique for encapsulating living organisms for generating active biological microthreads/scaffolds. Biomacromolecules 7:3364–3369
Wu J, Liu S, He L, Wang H, He C, Fan C, Mo X (2012) Electrospun nanoyarn scaffold and its application in tissue engineering. Mater Lett 89:146–149
Wu J, Huang C, Liu W, Yin A, Chen W, He C, Wang H, Liu S, Fan C, Bowlin GL, Mo X (2014) Cell infiltration and vascularization in porous nanoyarn scaffolds prepared by dynamic liquid electrospinning. J Biomed Nanotechnol 10:603–614
Wu S, Duan B, Qin X, Butcher JT (2017) Living nano-micro fibrous woven fabric/hydrogel composite scaffolds for heart valve engineering. Acta Biomater 51:89–100
Xue J, Niu Y, Gong M, Shi R, Chen D, Zhang L, Lvov Y (2015) Electrospun microfiber membranes embedded with drug-loaded clay nanotubes for sustained antimicrobial protection. ACS Nano 9:1600–1612
Acknowledgements
This work was supported by grants from National Natural Science Foundation of China (51503168), Shaanxi Innovation Talent Promotion Program-Project for Youth New Star of Science and Technology (2017KJXX-23), Special Funding for Postdoctoral Innovation Project in Shandong Province (201504), Disciplinary Construction Fund for Textile Science and Engineering of Xi’an Polytechnic University (10709-0821), and Xi’an Polytechnic University Innovation Fund for Graduate Students (CX201729).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Li, B., Liu, C., Zhou, F. et al. Preparation of electrospun core–sheath yarn with enhanced bioproperties for biomedical materials. Biotechnol Lett 40, 279–284 (2018). https://doi.org/10.1007/s10529-017-2466-3
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10529-017-2466-3