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Transcriptome analysis of Spodoptera frugiperda 9 (Sf9) cells infected with baculovirus, AcMNPV or AcMNPV-BmK IT

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Abstract

Objectives

To analyze the transcriptome of Spodoptera frugiperda 9 (Sf9) cells infected with AcMNPV or AcMNPV-BmK IT.

Results

A comprehensive transcriptome profile for Sf9 cells infected with AcMNPV or AcMNPV-BmK IT is shown. 43127572, 46529744 and 47235310 RNA-Seq profiles permitted the quantification of expression levels for control (C), AcMNPV-BmK IT treatment (ABT) and AcMNPV treatment (AT) groups. There were 997 up-regulated or down-regulated candidate genes with significant different expression level in these treatment groups.

Conclusion

These results provide a broad spectrum of candidate genes that are critically involved in the molecular regulation mechanism of Sf9 cells infected with AcMNPV-BmK IT.

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Acknowledgements

This project was supported by grants form National Natural Science Foundation of China for funding (No. 31272100, 31372199 and 31400765) and Natural Science Foundation of Shanxi Province (No. 2014011038-1). We thank Shuhua Zheng for the amendments of English. We also thank Sangon Biotech (Shanghai) Co., Ltd. for the transcriptome sequencing work.

Supporting information

Supplementary Table 1—Primers for qPCR.

Supplementary Table 2—Pollution test of C.

Supplementary Table 3—Pollution test of AT.

Supplementary Table 4—Pollution test of ABT.

Supplementary Table 5—Results of splicing.

Supplementary Table 6—Top ten up-regulated DEGs in AcMNPV and AcMNPV-BmK IT treatment compared to control.

Supplementary Table 7—Top ten down-regulated DEGs in AcMNPV and AcMNPV-BmK IT treatment compared to control.

Supplementary Table 8—Go and pathway enrichment of DEGS.

Supplementary Fig. 1—Analysis of three specimens.

Supplementary Fig. 2—KOG categories.

Supplementary Data 1—The RPKM quantification of DEGs in AcMNPV and AcMNPV-BmK IT treatment compared to control.

Supplementary Data 2—GO enrichment analysis for DEGs in AcMNPV and AcMNPV-BmK IT treatment compared to control.

Supplementary Data 3—KEGG enrichment analysis for DEGs in AcMNPV and AcMNPV-BmK IT treatment compared to control.

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Correspondence to Yuejun Fu.

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Wei, L., Cao, L., Miao, Y. et al. Transcriptome analysis of Spodoptera frugiperda 9 (Sf9) cells infected with baculovirus, AcMNPV or AcMNPV-BmK IT. Biotechnol Lett 39, 1129–1139 (2017). https://doi.org/10.1007/s10529-017-2356-8

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  • DOI: https://doi.org/10.1007/s10529-017-2356-8

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