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Colominic acid production from Escherichia coli in a fed-batch culture under the control of ammonium ions using an FIA system

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Abstract

A fed-batch culture was applied for the production of colominic acid from Escherichia coli. It was found that the copper ion, which is a trace element in medium and an essential element in N-acetylneuraminic acid synthase, affected the colominic acid production and the highest production was obtained using 1.6 mg/l CuCl2. Ammonia formed from nitrogen source utilization greatly affected colominic acid production and only slightly affected the cell growth. A flow injection analysis (FIA) system was fabricated and applied to monitor and control the ammonia concentration in the culture broth. It was found that a concentration of 0.3 g/l of ammonia was optimal for colominic acid production where 1.9 g/l was obtained.

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    Previously, we demonstrated that the biosynthesis of the PSA was inhibited as sorbitol level higher than 40 g/L [15]. Furthermore, it was observed that ammonia concentration could severely affect the cell growth and PSA production with E. coli, and a flow injection analysis (FIA) system was employed by Honda et al. to monitor and control the ammonia in an optimal level of 0.3 g/L, which resulted in a maximum PSA production of 1900 mg/L [16]. Hitherto, few reports on the isolation of PSA from the fermentation broth have been published impacting the industrial production of PSA.

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