화학공학소재연구정보센터
학회 한국공업화학회
학술대회 2010년 가을 (10/27 ~ 10/29, 대전컨벤션센터)
권호 14권 2호
발표분야 생물공학
제목 Cloning and expression of glycosyltransferase gene from a newly isolated Stenotrophomonas species
초록 Two types of glycosyltransferase genes from Stenotrophomonas sp. were cloned by PCR, and then inserted to pET-21b(+) expression vector. The recombinant GT genes were expressed in Escherichia coli and the crude cell extracts were reacted with flavonoid to analyze their abilities for glycosylation. We also analyzed the substrate specificity towards various flavonoids such as apigenin, chrysin, daidzein, genistein, kaempferol, luteolin, naringenin, resveratrol, quercetin and etc. with uridinediphosphate-activated glucose. The formation of flavonoids glucosides were analyzed and confirmed by HPLC.
저자 최성희1, 김희숙1, 이은열2
소속 1경성대, 2경희대
키워드 glycosyltransferase; flavonoid; uridinediphosphate-activated glucose
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