화학공학소재연구정보센터
Applied Chemistry for Engineering, Vol.28, No.1, 50-56, February, 2017
탈수소화효소 반응 및 촉매적 흡착 반응에 의한 액상 포름알데하이드의 제거 효율 연구
Investigation of Liquid Phase Formaldehyde Removal Efficiency by Enzymatic Formaldehyde Dehydrogenase and Catalytic Chemisorption Reactions
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초록
포름알데하이드는 무색, 무취의 유독성 물질로 다양한 방법을 통하여 포름알데하이드를 제거할 수 있는 방법들이 많이 보고되었다. 본 논문에서는 생물학적 효소 반응 및 화학적 흡착.촉매 반응에 의한 포름알데하이드의 제거 효율을 비활성을 통해 비교하였다. 첫째, Escherichia coli K12포름알데하이드 탈수소화 효소(Formaldehyde dehydrogenase, FDH)를 Escherichia coli BL21(DE3)에 클로닝하여 발현 및 분리하였다. FDH 효소 활성(kcat/Km)은 2.49 × 103 sec-1mM-1로 측정되었으며, 비활성은 8.69 U/mg으로 측정되었다. 둘째, 화학적 흡착 및 화학 촉매를 이용한 포름알데하이드의 제거 효율도 동시에 진행하였다. 본 논문에서는 활성탄과 제올라이트, KI 및 KOH로 처리한 활성탄과 제올라이트를 화학적 포름알데하이드 흡착제로 사용하였으며 Pd/TiO2 산화 촉매를 이용하여 포름알데하이드의 산화 반응 효율을 결정하였다. 결론적으로 본 논문에서 수행한 화학적 흡착 및 산화 촉매 반응의 경우 대략 50%의 비슷한 수준의 포름알데하이드 제거 효율을 보였으며, 특히 비활성의 경우 탈수소화효소의 비활성이 0.01-0.26 U/g 수준의 화학적 흡착 및 산화 촉매보다 월등히 높게 측정되었다.
Formaldehyde is one of the toxic substances without any color and smell. Several methods to remove formaldehyde has been investigated up to now. Here, both the enzymatic and chemisorptive/catalytic liquid phase formaldehyde removal were investigated, and their catalytic activities in terms of specific activities were compared. Firstly, formaldehyde dehydrogenase (FDH) enzyme from Escherichia coli K12 was cloned, and expressed in Escherichia coli BL21(DE3). And the catalytic activity was characterized as 2.49 × 103 sec-1mM-1 of kcat/Km with 8.69 U/mg of the specific activity. Secondly, the chemisorptive and oxidative catalytic removals were investigated simultaneously. Activated carbons and zeolites treated with heat, KI, and KOH were used as chemisorption medium. And Pd/TiO2 was used as an oxidative catalyst for the formaldehyde removal. All of the tested chemicals showed similar formaldehyde removal efficiencies of around 50%. However, the specific activity of FDH dependent formaldehyde removal was absolutely higher than that of using chemisorptive and catalytic removal processes with the ranges of 0.01 to 0.26 U/g.
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